A methanogen hydrolase reveals the structure of archaeal peptidoglycan – Nature


  • Vollmer, W., Blanot, D. & De Pedro, M. A. Peptidoglycan structure and architecture. FEMS Microbiol. Rev. 32, 149–167 (2008).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Albers, S.-V. & Meyer, B. H. The archaeal cell envelope. Nat. Rev. Microbiol. 9, 414–426 (2011).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Kandler, O. Zellwandstrukturen bei Methan-Bakterien. Naturwissenschaften 66, 95–105 (1979).

    Article 
    ADS 
    CAS 

    Google Scholar 

  • König, H., Kralik, R. & Kandler, O. Structure and modifications of pseudomurein in Methanobacleriales. Zentralblatt Für Bakteriol. Mikrobiol. Hyg. Abt Orig. C. 3, 179–191 (1982).

    Google Scholar 

  • König, H., Kandler, O., Jensen, M. & Rietschel, E. T. The primary structure of the glycan moiety of pseudomurein from Methanobacterium thermoautotrophicum. Hoppe. Seylers Z. Physiol. Chem. 364, 627–636 (1983).

    Article 
    PubMed 

    Google Scholar 

  • König, H. & Kandler, O. The amino acid sequence of the peptide moiety of the pseudomurein from Methanobacterium thermoautotrophicum. Arch. Microbiol. 121, 271–275 (1979).

    Article 
    PubMed 

    Google Scholar 

  • Kandler, O. & König, H. Chemical composition of the peptidoglycan-free cell walls of methanogenic bacteria. Arch. Microbiol. 118, 141–152 (1978).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • König, H. & Kandler, O. N-Acetyltalosaminuronic acid a constituent of the pseudomurein of the genus Methanobacterium. Arch. Microbiol. 123, 295–299 (1979).

    Article 

    Google Scholar 

  • Borrel, G., Brugère, J.-F., Gribaldo, S., Schmitz, R. A. & Moissl-Eichinger, C. The host-associated archaeome. Nat. Rev. Microbiol. 18, 622–636 (2020).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Hook, S. E., Wright, A.-D. G. & McBride, B. W. Methanogens: methane producers of the rumen and mitigation strategies. Archaea 2010, 945785 (2010).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Hoegenauer, C., Hammer, H. F., Mahnert, A. & Moissl-Eichinger, C. Methanogenic archaea in the human gastrointestinal tract. Nat. Rev. Gastroenterol. Hepatol. 19, 805–813 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Claus, H. & König, H. in Prokaryotic Cell Wall Compounds: Structure and Biochemistry (eds. König, H. et al.) 231–251 (Springer, 2010).

  • Alvarez, L., Cordier, B., Van Teeffelen, S. & Cava, F. Analysis of Gram-negative bacteria peptidoglycan by ultra-performance liquid chromatography. Bio-Protoc. 10, e3780 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Patel, A. V. et al. PGFinder, a novel analysis pipeline for the consistent, reproducible, and high-resolution structural analysis of bacterial peptidoglycans. eLife 10, e70597 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Luo, Y., Pfister, P., Leisinger, T. & Wasserfallen, A. The genome of archaeal prophage PsiM100 encodes the lytic enzyme responsible for autolysis of Methanothermobacter wolfeii. J. Bacteriol. 183, 5788–5792 (2001).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Pfister, P., Wasserfallen, A., Stettler, R. & Leisinger, T. Molecular analysis of Methanobacterium phage psiM2. Mol. Microbiol. 30, 233–244 (1998).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Leahy, S. C. et al. The genome sequence of the rumen methanogen Methanobrevibacter ruminantium reveals new possibilities for controlling ruminant methane emissions. PLoS One 5, e8926 (2010).

    Article 
    ADS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Luo, Y., Pfister, P., Leisinger, T. & Wasserfallen, A. Pseudomurein endoisopeptidases PeiW and PeiP, two moderately related members of a novel family of proteases produced in Methanothermobacter strains. FEMS Microbiol. Lett. 208, 47–51 (2002).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Weinberger, V. et al. Expanding the cultivable human archaeome: Methanobrevibacter intestini sp. nov. and strain Methanobrevibacter smithii ‘GRAZ-2’ from human faeces. Int. J. Syst. Evol. Microbiol. 75, 006751 (2025).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Turner, R. D. et al. Peptidoglycan architecture can specify division planes in Staphylococcus aureus. Nat. Commun. 1, 26 (2010).

    Article 
    ADS 
    PubMed 

    Google Scholar 

  • Wheeler, R., Mesnage, S., Boneca, I. G., Hobbs, J. K. & Foster, S. J. Super-resolution microscopy reveals cell wall dynamics and peptidoglycan architecture in ovococcal bacteria. Mol. Microbiol. 82, 1096–1109 (2011).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Blackman, S. A., Smith, T. J. & Foster, S. J. The role of autolysins during vegetative growth of Bacillus subtilis 168. Microbiology 144, 73–82 (1998).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Guo, L. et al. Insights into the catalytic mechanism of archaeal peptidoglycan endoisopeptidases from methanogenic phages. Int. J. Biol. Macromol. 296, 139672 (2025).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Makarova, K. S., Aravind, L. & Koonin, E. V. A superfamily of archaeal, bacterial, and eukaryotic proteins homologous to animal transglutaminases. Protein Sci. Publ. Protein Soc. 8, 1714–1719 (1999).

    Article 
    CAS 

    Google Scholar 

  • Xu, N., Huang, Z. H., de Jonge, B. L. & Gage, D. A. Structural characterization of peptidoglycan muropeptides by matrix-assisted laser desorption ionization mass spectrometry and postsource decay analysis. Anal. Biochem. 248, 7–14 (1997).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Glauner, B., Höltje, J. V. & Schwarz, U. The composition of the murein of Escherichia coli. J. Biol. Chem. 263, 10088–10095 (1988).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Petitjean, C., Deschamps, P., López-García, P., Moreira, D. & Brochier-Armanet, C. Extending the conserved phylogenetic core of archaea disentangles the evolution of the third domain of life. Mol. Biol. Evol. 32, 1242–1254 (2015).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Schleifer, K. H. & Kandler, O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36, 407–477 (1972).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Baquero, D. P. et al. Stable coexistence between an archaeal virus and the dominant methanogen of the human gut. Nat. Commun. 15, 7702 (2024).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Medvedeva, S., Borrel, G., Krupovic, M. & Gribaldo, S. A compendium of viruses from methanogenic archaea reveals their diversity and adaptations to the gut environment. Nat. Microbiol. 8, 2170–2182 (2023).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Vollmer, W., Joris, B., Charlier, P. & Foster, S. Bacterial peptidoglycan (murein) hydrolases. FEMS Microbiol. Rev. 32, 259–286 (2008).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Sham, L.-T., Barendt, S. M., Kopecky, K. E. & Winkler, M. E. Essential PcsB putative peptidoglycan hydrolase interacts with the essential FtsXSpn cell division protein in Streptococcus pneumoniae D39. Proc. Natl Acad. Sci. USA 108, E1061–1069 (2011).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Bartual, S. G. et al. Structural basis of PcsB-mediated cell separation in Streptococcus pneumoniae. Nat. Commun. 5, 3842 (2014).

    Article 
    ADS 
    CAS 
    PubMed 

    Google Scholar 

  • Fink, C. et al. The targeted deletion of genes responsible for expression of the mth60 fimbriae leads to loss of cell-cell connections in Methanothermobacter thermautotrophicus ΔH. Appl. Environ. Microbiol. 89, e00575-23 (2023).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Zeikus, J. G. & Wolfe, R. S. Methanobacterium thermoautotrophicus sp. n., an anaerobic, autotrophic, extreme thermophile. J. Bacteriol. 109, 707–715 (1972).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Wilson, S. A., Tank, R. K. J., Hobbs, J. K., Foster, S. J. & Garner, E. C. An exhaustive multiple knockout approach to understanding cell wall hydrolase function in Bacillus subtilis. mBio. 14, e0176023 (2023).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Rajguru, V., Chatterjee, S., Garde, S. & Reddy, M. Crosslink cleaving enzymes: the smart autolysins that remodel the bacterial cell wall. Trends Microbiol. 32, 494–506 (2024).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Egan, A. J. F., Errington, J. & Vollmer, W. Regulation of peptidoglycan synthesis and remodelling. Nat. Rev. Microbiol. 18, 446–460 (2020).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Pende, N. et al. SepF is the FtsZ anchor in archaea, with features of an ancestral cell division system. Nat. Commun. 12, 3214 (2021).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Ithurbide, S., Gribaldo, S., Albers, S.-V. & Pende, N. Spotlight on FtsZ-based cell division in Archaea. Trends Microbiol. 30, 665–678 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • van Wolferen, M., Pulschen, A. A., Baum, B., Gribaldo, S. & Albers, S.-V. The cell biology of archaea. Nat. Microbiol. 7, 1744–1755 (2022).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Rohs, P. D. A. & Bernhardt, T. G. Growth and division of the peptidoglycan matrix. Annu. Rev. Microbiol. 75, 315–336 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Garcia, P. S., Gribaldo, S. & Borrel, G. Diversity and evolution of methane-related pathways in archaea. Annu. Rev. Microbiol. 76, 727–755 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Bonin, A. S. & Boone, D. R. in The Prokaryotes (eds. Dworkin, M. et al.) 231–243 (Springer, 2006).

  • Thomas, C. M., Desmond-Le Quéméner, E., Gribaldo, S. & Borrel, G. Factors shaping the abundance and diversity of the gut archaeome across the animal kingdom. Nat. Commun. 13, 3358 (2022).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Pfeifer, K. et al. Archaea biotechnology. Biotechnol. Adv. 47, 107668 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Wheeler, R., Veyrier, F., Werts, C. & Boneca, I. G. Peptidoglycan and Nod receptor. In Glycoscience: Biology and Medicine 737–747 (Springer Japan, Tokyo, 2015).

  • Mistry, J. et al. Pfam: the protein families database in 2021. Nucleic Acids Res. 49, D412–D419 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Yu, N. Y. et al. PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes. Bioinformatics 26, 1608–1615 (2010).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Vranken, W. F. et al. The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59, 687–696 (2005).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Johnson, L. S., Eddy, S. R. & Portugaly, E. Hidden Markov model speed heuristic and iterative HMM search procedure. BMC Bioinf. 11, 431 (2010).

    Article 

    Google Scholar 

  • Katoh, K. & Standley, D. M. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol. Biol. Evol. 30, 772–780 (2013).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Criscuolo, A. & Gribaldo, S. BMGE (block mapping and gathering with entropy): a new software for selection of phylogenetic informative regions from multiple sequence alignments. BMC Evol. Biol. 10, 210 (2010).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Nguyen, L.-T., Schmidt, H. A., von Haeseler, A. & Minh, B. Q. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol. Biol. Evol. 32, 268–274 (2015).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Kalyaanamoorthy, S., Minh, B. Q., Wong, T. K. F., von Haeseler, A. & Jermiin, L. S. ModelFinder: fast model selection for accurate phylogenetic estimates. Nat. Methods 14, 587–589 (2017).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Hoang, D. T., Chernomor, O., von Haeseler, A., Minh, B. Q. & Vinh, L. S. UFBoot2: improving the ultrafast bootstrap approximation. Mol. Biol. Evol. 35, 518–522 (2018).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Capella-Gutiérrez, S., Silla-Martínez, J. M. & Gabaldón, T. trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25, 1972–1973 (2009).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Minh, B. Q. et al. IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era. Mol. Biol. Evol. 37, 1530–1534 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Letunic, I. & Bork, P. Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation. Nucleic Acids Res. 49, W293–W296 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 



  • Source link

    Leave a Reply

    Your email address will not be published. Required fields are marked *

    桜 (Sakura) 光 (Hikari) 未来 (Mirai) 空 (Sora) 希望 (Kibou) 星 (Hoshi) 絆 (Kizuna) 風 (Kaze) 夢 (Yume) 月 (Tsuki) 海 (Umi) 森 (Mori) 花 (Hana) 雨 (Ame) 虹 (Niji) 雪 (Yuki) 川 (Kawa) 太陽 (Taiyou) 雲 (Kumo) 山 (Yama) 愛 (Ai) 平和 (Heiwa) 自由 (Jiyuu) 旅 (Tabi) 心 (Kokoro) 勇気 (Yuuki) 情熱 (Jounetsu) 信義 (Shingi) 真実 (Shinjitsu) 奇跡 (Kiseki) 運命 (Unmei) 永遠 (Eien) 翼 (Tsubasa) 道 (Michi) 友 (Tomo) 家族 (Kazoku) 記憶 (Kioku) 時間 (Jikan) 宇宙 (Uchuu) 世界 (Sekai) 自然 (Shizen) 命 (Inochi) 朝日 (Asahi) 夕焼け (Yuuyake) 夜空 (Yozora) 銀河 (Ginga) 宝石 (Houseki) 静寂 (Seijaku) 感謝 (Kansha) 幸福 (Koufuku) 笑顔 (Egao) 響き (Hibiki) 波 (Nami) 潮風 (Shiokaze) 木漏れ日 (Komorebi) 黄昏 (Tasogare) 息吹 (Ibuki) 灯火 (Tomoshibi) 大地 (Daichi) 青空 (Aozora) 白雲 (Shirakumo) 清流 (Seiryuu) 翠雨 (Suiu) 春風 (Harukaze) 秋桜 (Kosumosu) 冬景色 (Fuyugeshiki) 夏空 (Natsuzora) 星座 (Seiza) 流星 (Ryuusei) 満月 (Mangetsu) 新月 (Shingetsu) 暁 (Akatsuki) 黎明 (Reimei) 陽光 (Youkou) 紫陽花 (Ajisai) 向日葵 (Himawari) 紅葉 (Momiji) 銀杏 (Ginkgo) 朝露 (Asatsuyu) 薫風 (Kunpuu) 初雪 (Hatsuyuki) 蛍火 (Hotarubi) 泡沫 (Utakata) 悠久 (Yuukyuu) 天の川 (Amanogawa)